Evaluation of Mosquito Attractant Candidates Using a High-Throughput Screening System for Aedes aegypti (L.), Culex quinquefasciatus Say. and Anopheles minimus Theobald (Diptera: Culicidae)
Evaluation of Mosquito Attractant Candidates Using a High-Throughput Screening System for Aedes aegypti (L.), Culex quinquefasciatus Say. and Anopheles minimus Theobald (Diptera: Culicidae)
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Several types of olfactometers have been used to evaluate mosquito responses to agents that mimic natural volatiles that repel or attract.The Y-tube olfactometer has been widely used to study repellents and attractants, while the high-throughput screening system assay has only been used to study repellents.Whether the high-throughput screening system assay is suitable for evaluating attractants is unknown.
We evaluated the responses to four lactic-acid-based mixtures and two non-lactic-acid-based chemical lure candidates using the high-throughput screening system (HITSS) for three mosquito species quest fryer (laboratory strains and field populations of both Aedes aegypti (L.) and Culex quinquefasciatus Say.; laboratory strain of Anopheles minimus Theobald) under laboratory-controlled conditions.
HITSS assay results showed that KU-lure #1 elicited the greatest percent attraction for pyrethroid-resistant and -susceptible Ae.aegypti.KU-lure #6 elicited the strongest attractive response for pyrethroid-susceptible and -resistant Cx.
quinquefasciatus and pyrethroid-susceptible An.minimus.The response to the lures from each species was independent of the pyrethroid susceptibility status (Ae.
aegypti, p = 0.825; Cx.quinquefasciatus, p = 0.
056).However, a significant difference in attraction to KU-lure #6 was observed metabo 15-gauge finish nailer cordless between diurnal and nocturnal mosquitoes (Cx.quinquefasciatus vs.
Ae.aegypti, p = 0.014; An.
minimus vs.Ae.aegypti, p = 0.
001).The laboratory-level HITSS assay effectively selects potential lure candidates.Because the host-seeking behavior differs between mosquito species, further studies are needed to develop species-specific attractants.
Additional studies in semi-field screen houses using commercial traps are necessary to evaluate the accuracy of these laboratory assay results.